Improving Fluorescence Imaging with Laser Illumination
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DAPI Fluorophore: Laser + Filter Selection Guide
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FITC Fluorophore: Laser + Filter Selection Guide
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Texas Red Fluorophore: Laser + Filter Selection Guide
Coherent® High Performance OBIS™ Laser Systems
264 +44 (0) 1904 788600 | Edmund Optics® N NEW PRODUCT NEW LOW PRICE
testing & detection targets illumination cameras imaging lenses microscopy lasers optomechanics optics
Coherent® High Performance OBIS™ LX/LS Laser Systems
• Same Compact Design for All Wavelength Options
• Integrated Control Electronics with Analog and Digital Modulation
• Circular Beam with Superior Beam Quality
Coherent® High Performance OBIS™ LX/LS Laser Systems are compact, plug-and-play lasers that o er a
wide range of wavelengths from the ultraviolet to the near-infrared in a single platform. Although each laser
utilizes one of two technologies, either Coherent’s proprietary Optically Pumped Semiconductor Laser
(OPSL) or laser diode-based, these laser systems feature the same beam parameters in packages that allow
the user to plug-and-play alternate wavelengths as needed. Coherent® High Performance OBIS™ LX/LS Laser
Systems includes a USB, RS-485, and full I/O interface. A multicolor LED has been integrated into the top
cover to provide laser status at a glance. Note: A power supply is required for operation and sold separately.
OBIS remote is required for CDRH certi ed systems. The OBIS heat sink is recommended.
LASER RADIATION
AVOID DIRECT EXPOSURE
TECHNICAL NOTE
System performance can be improved by using two lters: one between the
illumination source and the sample and a second between the sample and
the detector. The purpose of ltering is to limit all light to within the excitation
or emission bands. System performance can be further improved by
using a narrow band illumination source that is fully contained within an
excitation band, like a laser, eliminating the need for an excitation lter.
The gures below give examples of popular uorophores and match
an appropriate illumination laser and emission lter that could be used
within a high performance uorescence imaging or microscopy system.
The graphs below show the location of excitation and emission bands for
three common uorophores and highlight speci c lters and lasers that can
be used together to isolate the peak transmission from the emission bands.
0
300 325 350 375 400 425 450 475 500 525 550 575 600
Transmission (%) Relative Intensity (%)
Wavelength (nm)
Excitation
Emission
Filter (#84-095)
Laser (#87-453)
0
350 375 400 425 450 475 500 525 550 575 600 625 650
Transmission (%) Relative Intensity (%)
Wavelength (nm)
Excitation
Emission
Filter (#67-031)
Laser (#87-459)
0
450 475 500 525 550 575 600 625 650 675 700 725 750
Transmission (%) Relative Intensity (%)
Wavelength (nm)
Excitation
Emission
Filter (#67-035)
Laser (#12-355)
Wavelength
(nm)
Output
Power
(mW)
Mode
Quality,
M2
Beam
Diam.
@ 1/e2
(mm)
Digital
Modulation
(MHz)
Analog
Modulation
(KHz)
Operating
Temp
(°C)
Coherent
Model
No.
Stock
No. Price
375 16 1,3 0,7 100 450 10 to 50 1185045 #87-453 €8.550,00
375 50 1,3 0,7 100 450 10 to 50 1185046 #87-454 €10.975,00
405 50 1,2 0,8 150 500 10 to 50 1185048 #87-455 €4.625,00
405 100 1,2 0,8 150 500 10 to 50 1185049 #87-456 €5.145,00
405 140 1,3 0,8 150 500 10 to 50 1265576 #12-336 €5.995,00
405 200 1,3 0,8 150 500 10 to 40 1185050 #88-022 €8.550,00
405 250 1,3 0,8 150 500 10 to 50 1303774 #12-337 €9.220,00
422 100 1,2 0,9 150 500 10 to 50 1224333 #12-338 €8.385,00
445 75 1,2 0,6 150 500 10 to 50 1185051 #87-457 €7.300,00
458 75 1,2 0,8 150 500 10 to 50 1228315 #12-339 €7.090,00
473 75 1,2 0,7 150 500 10 to 50 1185052 #87-458 €7.250,00
488 20 1,2 0,7 0,05 100 10 to 40 1178767 #87-459 €5.550,00
488 50 1,2 0,8 150 500 10 to 50 1185053 #87-460 €6.350,00
488 60 1,1 0,7 0,05 100 15 to 40 1178769 #88-023 €6.175,00
488 80 1,1 0,7 0,05 100 15 to 40 1230933 #88-024 €7.500,00
488 100 1,1 0,7 0,05 100 15 to 40 1226419 #88-025 €8.125,00
488 150 1,1 0,7 0,05 100 15 to 40 1277611 #12-340 €8.100,00
488 150 1,2 0,8 150 500 10 to 50 1220123 #87-461 €8.400,00
505 50 1,2 0,7 150 500 10 to 50 1270955 #12-341 €7.090,00
514 20 1,1 0,7 0,05 100 10 to 40 1178771 #87-462 €5.700,00
514 40 1,2 0,6 100 500 10 to 50 1202821 #12-342 €3.895,00
520 40 1,2 0,6 100 500 10 to 50 1202826 #12-343 €3.895,00
532 20 1,1 0,7 0,05 500 15 to 40 1261777 #33-350 €4.975,00
532 50 1,1 0,7 0,05 100 15 to 40 1261779 #12-344 €5.900,00
532 80 1,1 0,7 0,05 100 15 to 40 1264453 #12-346 €7.150,00
Wavelength
(nm)
Output
Power
(mW)
Mode
Quality,
M2
Beam
Diam.
@ 1/e2
(mm)
Digital
Modulation
(MHz)
Analog
Modulation
(KHz)
Operating
Temp
(°C)
Coherent
Model
No.
Stock
No. Price
532 100 1,1 0,7 0,05 100 15 to 40 1261781 #12-347 €8.400,00
532 150 1,1 0,7 0,05 100 15 to 40 1280718 #12-348 €9.400,00
552 20 1,1 0,7 0,05 100 10 to 40 1178779 #87-463 €5.975,00
552 60 1,1 0,7 0,05 100 15 to 40 1230937 #12-349 €6.850,00
552 80 1,1 0,7 0,05 100 15 to 40 1230939 #12-350 €8.950,00
552 100 1,1 0,7 0,05 100 15 to 40 1230941 #12-351 €9.650,00
552 150 1,1 0,7 0,05 100 15 to 40 1284009 #12-352 €11.200,00
561 20 1,1 0,7 0,05 100 15 to 40 1223779 #88-026 €6.050,00
561 50 1,1 0,7 0,05 100 15 to 40 1230935 #88-027 €7.050,00
561 80 1,1 0,7 0,05 100 15 to 40 1253299 #12-353 €8.950,00
561 100 1,1 0,7 0,05 100 10 to 40 1253301 #34-232 €9.950,00
561 150 1,1 0,7 0,05 100 15 to 40 1280720 #12-354 €11.200,00
594 20 1,1 0,7 0,05 100 15 to 40 1233468 #12-355 €6.510,00
594 60 1,1 0,7 0,05 100 15 to 40 1233470 #12-356 €8.110,00
594 100 1,1 0,7 0,05 100 15 to 40 1285743 #34-233 €11.200,00
637 140 1,2 0,7 150 300 10 to 50 1196625 #87-464 €3.085,00
640 40 1,2 0,8 150 500 10 to 50 1185054 #87-465 €2.055,00
640 100 1,2 0,8 150 500 10 to 50 1185055 #87-466 €2.575,00
647 120 1,2 0,8 150 500 10 to 50 1196627 #87-467 €3.475,00
660 100 1,2 0,9 150 500 10 to 50 1185057 #87-468 €1.650,00
685 40 1,2 0,8 150 500 10 to 50 1202346 #87-469 €2.050,00
730 30 1,2 0,8 150 500 10 to 50 1196629 #87-470 €3.500,00
808 150 1,2 0,7 150 500 10 to 50 1314237 #12-357 €3.395,00
980 100 1,3 0,7 150 500 10 to 50 1313871 #12-358 €2.495,00
980 150 1,3 0,7 150 500 10 to 50 1333336 #12-359 €3.395,00
Visit edmundoptics.eu/laser-optics to view our entire selection of laser products
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